Lab Eph 2: Fill & Download for Free

Thats a good question,. Sorry to see mockers running their mouths at you.Even worse is the advise to see a doctor. Psychiatrists are the upper limit of hypocrisy. Sorcerers as mentioned on the last page of your Bible. They dont know how the psych meds they deal work on the human brain. Witch doctors playing lab rat with your Spirit, They know its its evil to chemically bomb someones brain. They don’t care.Anyway here's your Answer.Absolutely Satan possess ppl.God made Satan perfect in beauty and wisdom. He was our protector, Was An anointed Cherub covering the alter of God. He has a lot of pull and a lot of entities follow him.Revelation : ‘…..The whole world wonders after Satan..’Eph 2 . Satan “…the Prince of the air..’ Eph 6 We wrestle and fight a constant deliberate targeted beat down. Not from flesh and Blood. The fight is against Powers and rulers of darkness in this world. Against spiritual wickedness in high places.Satans has an army of Demons, Devils, Evil Spirits, and many hidden powers and principalities.Satan has 4 dynasties brutalizing mankind that can be seen escalating right now. 1) Mainstream Religions 2)WorldWide Politics/Political systems 3) WorldWide Economy 4)The Multi-Media Education/Information social engineering system.There are ways to protect yourself. Ask me.Read this carefully 2Cor Ch:11 V:2

PERVs or porcine endogenous retroviruses are present in the nuclei of all pig cells so avoiding their transfer during xenotransplantation is practically impossible (1). If humans did get infected with PERVs from a xenotransplant, they'd need to get treated, same as with other retroviral infections.Rx with standard anti-retroviral drugs would be an obvious choice but which ones are effective against PERVs?Among Reverse-transcriptase inhibitor used in humans, azidothymidine (Zidovudine) showed anti-PERV activity (2, 3). However, since azidothymidine is also quite toxic, it may not be realistically feasible, i.e., safe enough, for someone who's also simultaneously undergoing immunosuppression, as patients with transplants do, or if used, it may be necessary to use at lower doses, in which case it may not be completely effective.Among newer anti-retrovirals, Discovery and development of integrase inhibitors such as Raltegravir (4) and Dolutegravir (3) have shown potent anti-PERV activity in vitro.Decades of experience in HIV also show that multi-drug therapy, i.e., using anti-retroviral drugs that target different viral pathways, is the optimal approach since it increases efficacy and reduces risk of drug resistance.The cutting-edge Genome editing approach, specifically the CRISPR system can inactivate multiple PERV loci (5). This important proof-of-concept suggests it may be possible to proactively remove PERVs from pig graft tissue prior to transplanting into humans.It's also important to assess the likelihood of actual PERV transmission and there's data to do so since >200 individuals have received some form of pig transplants (mainly pancreatic islets) or exposure (mainly to pig kidney, liver, neuronal or skin cells) in a variety of experimental studies. So far, there's not a single report of PERV transmission to humans among these recipients (6, 7, see list below from 8).However, in vitro data from more than one lab shows that PERVs can be transmitted in human cells exposed to pig-derived blood, plasma or cells in culture (9, 10, 11, 12). Thus, though risk to humans is reportedly low (1, 10), nevertheless it's tangible enough that it needs to be addressed with defined and rational scientific approaches (13, see quote below).'In general, it is likely that the development of surveillance techniques might be guided by the ‘‘Precautionary Principle.’’ That is, 'the risk of xenogeneic infection is generally thought to be low but the deployment of appropriate procedures and assays should not wait until a risk is confirmed' (World Health Organization. OECD/WHO Consultation on Xenotransplantation Surveillance—WHO. CDS/CSR/EPH/2001.2 (Guidance Document), 2000)'Bibliography1. Takeuchi, Yasuhiro, and Jay Fishman. "Long life with or without PERV." Xenotransplantation 17.6 (2010): 429-430.2. Qari, Shoukat H., et al. "Susceptibility of the porcine endogenous retrovirus to reverse transcriptase and protease inhibitors." Journal of virology 75.2 (2001): 1048-1053. Susceptibility of the Porcine Endogenous Retrovirus to Reverse Transcriptase and Protease Inhibitors3. Argaw, Takele, Winston Colon‐Moran, and Carolyn Wilson. "Susceptibility of porcine endogenous retrovirus to anti‐retroviral inhibitors." Xenotransplantation 23.2 (2016): 151-158.4. Demange, Antonin, et al. "Porcine endogenous retrovirus-A/C: biochemical properties of its integrase and susceptibility to raltegravir." Journal of General Virology 96.10 (2015): 3124-3130.5. Yang, Luhan, et al. "Genome-wide inactivation of porcine endogenous retroviruses (PERVs)." Science 350.6264 (2015): 1101-1104. http://arep.med.harvard.edu/pdf/Yang_Science_2015.pdf6. Heneine, Walid, et al. "No evidence of infection with porcine endogenous retrovirus in recipients of porcine islet-cell xenografts." The Lancet 352.9129 (1998): 695-699.7. Dinsmore, Jonathan H., et al. "NO EVIDENCE FOR INFECTION OF HUMAN CELLS WITH PORCINE ENDOGENOUS RETROVIRUS (PERV) AFTER EXPOSURE TO PORCINE FETAL NEURONAL CELLS1." Transplantation 70.9 (2000): 1382-1389.8. Denner, Joachim. "Xenotransplantation-Progress and Problems: A Review." Journal of Transplantation Technologies & Research 2014 (2014). http://www.omicsonline.org/pdfdownload.php?download=open-access/xenotransplantationprogress-and-problems-a-review-2161-0991.1000133.pdf&aid=287029. Patience, Clive, Yasuhiro Takeuchi, and Robin A. Weiss. "Infection of human cells by an endogenous retrovirus of pigs." Nature medicine 3.3 (1997): 282-286.10. Le Tissier, Paul, et al. "Two sets of human-tropic pig retrovirus." Nature 389.6652 (1997): 681-682.11. Wilson, Carolyn A., et al. "Type C retrovirus released from porcine primary peripheral blood mononuclear cells infects human cells." Journal of virology 72.4 (1998): 3082-3087. Type C Retrovirus Released from Porcine Primary Peripheral Blood Mononuclear Cells Infects Human Cells12. Wood, James C., et al. "Identification of exogenous forms of human-tropic porcine endogenous retrovirus in miniature swine." Journal of virology 78.5 (2004): 2494-2501. Identification of Exogenous Forms of Human-Tropic Porcine Endogenous Retrovirus in Miniature Swine13. Fishman, Jay A., Linda Scobie, and Yasuhiro Takeuchi. "Xenotransplantation‐associated infectious risk: a WHO consultation." Xenotransplantation 19.2 (2012): 72-81. https://www.researchgate.net/profile/Linda_Scobie/publication/224004146_Xenotransplantation-associated_infectious_risk_a_WHO_consultation/links/5538db010cf2239f4e79c2b3.pdfThanks for the R2A, Jai Padmakumar.

I’ve spent much of my lifetime in school (MIT, Einstein, Caltech, Harvard) and I’ve had some wonderful teachers along the way. And this question is a great opportunity to exercise gratitude, which, btw, is a good way to start the New Year. So here’s my chance to say thank you to my most amazing teachers:My father. He was a world famous medical scientist (Elvio Sadun - Wikipedia) and taught me something every evening when we would take a walk after dinner. But, perhaps most importantly, he taught me that you can learn from almost anyone by being a careful listener. He never missed an opportunity. We’d take cab rides when in NYC and he’d get the cab driver to talk about himself and then later analyze, for me, what the man had to teach. And he never criticized me for failing, only for being uncharitable in my judgments of myself or others.My mother. She was very critical and always expected better. It’s useful to have someone like that around, especially if you’re an arrogant teenager. If I got a 98 on a test in school, she’d obsess over the 2 points I missed. I grew up thinking she wasn’t proud of me, and only learned, decades later, that she did brag about me, but only behind my back. It’s an old school thing.Vicki Weisskopf at MIT. World famous for his contributions in quantum mechanics and QED, and chairman of the department of physics, he still loved teaching personally. His one lesson for me was stated thusly: “To be a great teacher, you need to lie a little. 98% of the truth suffices for undergraduates. Save 1% for the graduate students and 1% for professors.” By making things just a tiny bit simpler, it became possible for many of us to better understand deep truths. But it took genius to know which 2% to leave out.Philip Morrison at MIT. Phil was a small man crippled by polio. But he was a giant when he talked about science. I loved his wonderful stories about the physics of the atom bomb developed at the Manhattan Project at Los Alamos. He wrote book reviews for the journal SCIENTIFIC AMERICAN every month and reading all this science made him encyclopedic. The best part is that he brought together all this different science, as in his book, THE RING OF TRUTH The Ring of Truth - YouTubeWalle Nauta at MIT. A very gentle scholar of the old school, Nauta taught neuro-anatomy with a Dutch accent for graduate students. I made the mistake of taking his class as an undergraduate and almost failed it. But he wouldn’t let me. He helped me with my homework and was amazingly generous with his time and attention. I still don’t understand why. And a shout out to Jerry Schneider, a young assistant professor in the department, who arranged to let me have my own tiny little lab and my own funding while still an undergraduate at MIT.Dominic Purpura at Einstein. Chairman of the Department of Neuroscience and one of my thesis advisers. Dom worked with a frenzy. He was editor-in-chief of the journal, Brain Research, so he knew everything in the field. But when I had to dissect brains (of several animal model species), he would be there personally to help find all the delicate parts. He knew everything and he always had time. Later, I dropped by his office while he was Dean of Stanford Medical School, and he still had time to discuss, with enthusiasm, subtle brain pathways, just discovered.Pat Model at Einstein. She was the only woman in the department and another thesis adviser (a shout out to my third adviser, George Pappas, a kind and encouraging man). But while George was gentle, Pat was in your face. She was the one who told me, just before I did my doctoral thesis defense, that sex wouldn’t get better for me after I got the PhD. Just like that, and in public. But she had a bigger point to make, and it stuck. Your accolades accumulate, but you remain you.Ephraim Freedman at Einstein and Harvard. Eph was the dean at Einstein. I only got to know him after he read my PhD thesis and asked me what I wanted to do with my life after I got my MD. He figured out that though I didn’t even know the existence of the field of Neuro-Ophthalmology, it would be a perfect fit for me. So to help me decide, he arranged for me to do an away rotation with the world’s premier Neuro-Ophthalmologist. It must have cost him a lot of political equity to pull it off. Later, he was my chief at Mass. Eye and Ear (Harvard), and I asked him why he did it. He said he figured I’d pay it forward later. I’m still trying.Claes Dohlman at Harvard. Claes was the Chairman of Ophthalmology. He also was a gentleman and scholar of the old school. He took me into the OR to do a case for him when I was the most inexperienced resident and, when things started to go south, I turned the instruments over to him. He refused them saying that he who gets into a jam has to learn how to get out on his own. After that, I did all my surgeries with much greater confidence knowing that Claes trusted in me that I could always find a way out.Ken Kenyon at Harvard. Ken was chief of cornea, super ambitious and super bright. But his lesson for me was in how to elevate every discussion, every case, every introduction to a higher level. In treating people with generosity and great respect, he raised everyone’s game.Simmons Lessell at Harvard. Simmons taught me almost everything I know in Neuro-Ophthalmology and a lot about being “a doctor”. He was the consummate clinician and also the consummate teacher. He combined my mother’s intensity and attention to detail with Weisskopf’s genius for teaching, all in the intimacy of a one-on-one fellowship. We spent 12 hours a day, 5 days a week within 10 feet of each other, over the course of a year, with another lesson coming every few minutes.Ron Smith at USC. Ron was a cornea guy from Hopkins who became the Chairman of Ophthalmology at Doheny/USC. I arrived as his opposite in many senses, but for some reason we developed a deep bond and spent a lot of time together. Eventually, I became the Vice-Chair and we spent hours every day planning for our department. Ron was always quick to share credit and promote others. Perhaps his best lesson was, “The world belongs to those that show up.”Only three of the dozen people above are still alive. However, I hope that like all great teachers, their legacy will continue through their students who were made much better by the teacher’s brilliant efforts and inspiration. Teachers, with minimal drama, change the world the most.