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A Stepwise Guide in Editing a Rosbreed on Mac

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PDF Editor FAQ

Can fruit DNA (like from an Apple or orange) be traced back to the tree it came from?

Yes, but it can be easy or hard.Pedigree records and germplasm management (i.e. maintaining historical stocks) are key. If you have accurate records and reliable stocks, this is pretty easy.Without accurate pedigree records - it can still be done, but it requires a lot of markers and you’ll still need historical stocks. Your accuracy will be off.I’ve seen examples of both. The Honeycrisp apple is a great example. Part of the pedigree was known, but part of it was wrong (as determined by molecular DNA markers). If you read the abstract, they’ve since been able to verify a number of the grandparents, but one of the parents is now lost. However, they are now able to make an educated guess as to the genetic makeup of that parent.And thanks for asking the question, looks like there was a 2017 update that I wasn’t aware of.The apple (Malus×domestica) cultivar Honeycrisp has become important economically and as a breeding parent. An earlier study with SSR markers indicated the original recorded pedigree of ‘Honeycrisp’ was incorrect and ‘Keepsake’ was identified as one putative parent, the other being unknown. The objective of this study was to verify ‘Keepsake’ as a parent and identify and genetically describe the unknown parent and its grandparents. A multi-family based dense and high-quality integrated SNP map was created using the apple 8 K Illumina Infinium SNP array. This map was used alongside a large pedigree-connected data set from the RosBREED project to build extended SNP haplotypes and to identify pedigree relationships. ‘Keepsake’ was verified as one parent of ‘Honeycrisp’ and ‘Duchess of Oldenburg’ and ‘Golden Delicious’ were identified as grandparents through the unknown parent. Following this finding, siblings of ‘Honeycrisp’ were identified using the SNP data. Breeding records from several of these siblings suggested that the previously unreported parent is a University of Minnesota selection, MN1627. This selection is no longer available, but now is genetically described through imputed SNP haplotypes. We also present the mosaic grandparental composition of ‘Honeycrisp’ for each of its 17 chromosome pairs. This new pedigree and genetic information will be useful in future pedigree-based genetic studies to connect ‘Honeycrisp’ with other cultivars used widely in apple breeding programs. The created SNP linkage map will benefit future research using the data from the Illumina apple 8 and 20 K and Affymetrix 480 K SNP arrays.

Can Bing cherry branches be grafted onto a wild cherry tree?

Sure, you can try. Actually, “Bing”’ cherries are not grown from seed, but instead must be grafted to create the perfect fruit. There were several trees involved in the creation of the original Bing cherry and several others are needed to continue reproducing it.Bing cherries originated in 1875 at Henderson Lewelling's farm. Their namesake was Ah Bing, the Chinese foreman at Lewelling's farm. It is unclear whether Bing was the creator of the cherry, or if naming it was a tribute to a favorite worker. For years it was only known that the mother tree was a grafted Black Republican cherry, but the father was unknown. Scientists at the RosBREED project announced in 2013 that they had finally determined the father cherry and pollen donor to be the Napoleon Bigarreau.Since growing a perfect Bing cherry tree from seed is not possible, propagation requires grafting. You need scions, or small branches of new growth, from a donor Bing tree and rootstock to graft the scions onto. Mahaleb cherry, (Prunus mahaleb) is the most common rootstock choice. It is easy and fast to grow, and slightly dwarfing. Its major downfall is that it can be susceptible to rot and fungal diseases. Another good choice is Mazzard, (Prunus avium "Mazzard") which is slow to fruit but grows much larger than Mahaleb and makes larger cherries. Both varieties are well suited to USDA zones 5 to 8 just like the Bing.Source: What Two Cherry Trees Are Needed to Produce Bing Cherries?

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