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I would love to see Amazon give Saint Louis serious consideration. I'm a New York Native who moved to Saint Louis three years ago. I've worked in hi-tech since graduating from college, and I currently work as a Senior Software Development Manager. Over the years, I've filed a number of patents in the areas of IoT and Big Data, among others. Amazon has invited me to interview 12 times in the last 24 months, and while working for Amazon is high on my bucket list, I'm not willing to give up the lifestyle of Saint Louis and move to a New York or Seattle. Here's why:My commute to work is 5 minutes. Parking my car and taking the elevator to my office takes longer than the drive does. In fact, my whole life is within a 5 minute drive from my home. My kids school, our shopping and doctors. I've given up going to the Costco that's 15 minutes away because it's too far. I buy my larger items on Amazon Prime instead.The cost of living in Saint Louis is 60% less than NYC, 40% less than Boston, 35% less than Seattle and 11% less than Dallas. The average house costs $120,000. There aren't many metropolitan areas where you can buy a 3,000 square-foot-home for $350,000. In Saint Louis you can. We also have a newly revamped downtown with networks of lofts, bars, and cafes, offering residents the ability to walk or bike to work without paying big-city prices. These may not seem like a big deal for many Midwest natives, but for those of us who only know the nose-bleed real estate prices and cramped conditions found in big coastal cities, an affordable and livable region like Saint Louis seems like a revelation. It’s not only my husband and I who have embraced this simple fact. Since moving to Saint Louis, we've convinced my husband's brother as well as my sister and her family to move to Saint Louis from Miami and NYC respectively. And to be honest, it wasn't a hard sell.And it’s not just affordable housing that has sold us on Saint Louis. Recreation and entertainment here are on par with any major city. My family spends weekends at the Saint Louis Zoo (free), the Science Center (free), the Botanical Garden (free), the History Museum (free, and more fun than you'd think) and The Magic House (annual membership $80). We go skiing and snow tubing a half hour away and Six Flags Saint Louis is as much fun as I remember the one in New Jersey being. Forest Park, at the heart of the city, offers a panorama of green, and at 1300 acres it is 500 acres larger than New York's Central Park. Over the summer months, you can watch shows outdoor at The Muny, America's oldest and largest outdoor theatre. As the home of Anheuser-Busch, we have a unique and venerable culture of craft breweries and beer gardens. We also have professional sports year round, and now that the Rams have moved to LA, all of our sports teams are first class. The Cardinals and the Blues are teams that anyone can be proud of.A lot has been written about the food culture in Saint Louis and we have a number of unique culinary styles. We have our own style of regional barbecue, frozen custard and gooey butter cake. And Imo's is famous for serving up authentic, square sliced, provel covered Saint Louis pizza. Although I keep strictly kosher and have limited experience eating out, I've heard that Reeds American Table has awesome farm to table fancy American style food. Nora's is often on lists for best sandwiches in the country. Hi-Pointe Drive-In offers awesome burgers and shakes in a building made out of shipping containers. And while I can't speak to these personally, I can attest to the fact that Kohn's Kosher Deli is on par with any NYC deli. It's more affordable as well.Saint Louis is small enough that Amazon's presence will make a difference, and big enough that you won't be the only player in town. Monsanto, Emerson and Enterprise, among others, call Saint Louis home. And with Centene and Express Scripts based here in Saint Louis, there's a great supply of health care talent to tap into. Beginning in 2011 Saint Louis has been growing its entrepreneurial footprint. Since then, Arch Grants was founded and the CIC made Saint Louis it's first expansion outside Massachusetts. Today there's a great start up culture in the Central West End and downtown. Venture Cafemeetups at the CIC on Duncan Street help drive innovation across industries in the city. We're the birthplace of Launch Code and StrangeLoop. And Walter Sobchak was born here as well.Saint Louis is also the hometown of Jack Dorsey, who is one of the few start up founders in recent memory to have successfully launched two technology companies and taken both public. And when Dorsey’s Square was looking for a place to expand beyond its San Francisco digs, the payment processor chose Saint Louis, both for its affordability and great talent pool, thanks to a large local presence in the financial services industry. Square’s offices in the Central West End are right next to a construction site where a new office building is under construction. And guess who will soon be taking up space in that office? Your neighbor from Redmond, Microsoft. It seems to me Amazon would be in good company here. There is also the small matter of Jacob Mick, whom you lured away. We’d like to see him come home.I moved to Saint Louis not sure what to expect. In the three years that I've been here I've found friends, a lifestyle that's hard to give up, and today I'm proud to call Saint Louis home. Saint Louis has the benefits of a big city and the charm, culture and prices of a small city. Saint Louis is rich with history and existing infrastructure, and still at the cutting edge of technology, lifestyle, and development. I think that Amazon can build a strong HQ2 in Saint Louis, and that Amazon employees would consider themselves lucky to live here as well. Plus, it doesn't rain that much here.Do you want to see Saint Louis become home to Amazon's HQ2? Like and share if you do.

The Human microbiota. Everyone has a slightly different mix of bugs that live in us and on us. They out number the cells of our body and perform many critical functions that are not well understood. They are as intimate to who we are as our own blood but we know very little about the role they play other than we could not live without them.The Elizabeth H. and James S. McDonnell III Genome Institute at Washington UniversityOur bodies are teeming with life – trillions of microbes occupy virtually every surface – inside and out. They even outnumber our own cells ten to one. And much like our planet has its ecosystem of plants and animals interacting with their environments, we have our own microscopic one filled with living organisms that inhabit our bodies’ diverse environments.“Our bodies are part of a microbial world,” says Dr. George Weinstock, associate director of The Genome Institute. “You can think of our ecosystems like you do rain forests and oceans, very different environments with communities of organisms that possess incredible, rich diversity.”A Microbial EcosystemMost of the time this microbial ecosystem coexists peacefully with us. Many of our microbes help us digest food, strengthen our immune systems and even protect us from dangerous pathogens. But it has proven very difficult to study many of these microbes, which differ depending on body site and individual. The microbes are also difficult to isolate and grow in the lab. As a result, researchers have understood little about which microbes reside in specific sites of the body. Now, a consortium of some 200 U.S. scientists at Washington University School of Medicine in St. Louis and elsewhere report findings from the most comprehensive census of the microbial makeup of healthy humans.Published June 14 in Nature and in several Public Library of Science (PLoS) journals, the research offers new details and even some surprises.Know Thy MicrobesFor example, the researchers found that even healthy people typically carry low levels of harmful bacteria in and on their bodies. But when a person is healthy, these pathogens don’t cause disease; they simply coexist in an abundance of beneficial microbes. Now, scientists can investigate why some pathogens can suddenly turn deadly, an endeavor that will refine current thinking on how microorganisms cause disease.“It’s not possible to understand human health and disease without exploring the massive community of microorganisms we carry around with us,” says Dr. Weinstock, one of the project’s principal investigators. “Knowing which microbes live in various ecological niches in healthy people allows us to better investigate what goes awry in diseases that are thought to have a microbial link, like Crohn’s, acne, periodontitis, vaginosis, or urethritis, and why dangerous pathogens sometimes, but not always, cause life-threatening illnesses.”Human Microbiome ProjectThe Genome Institute played a major role in the new research, known as the Human Microbiome Project. The five-year initiative was funded with $153 million from the National Institutes of Health (NIH), with some $32 million coming to the university. TGI scientists decoded about half of the 5,000 specimens from nearly 250 healthy volunteers.To get a handle on the healthy human microbiome, the researchers sampled 15 body sites in men and 18 in women, including areas of the mouth (nine sites, including the teeth), skin (two behind the ear and each inner elbow), nose, vagina (three sites) and lower intestine (stool). In St. Louis, most samples were collected from study participants enrolled at the School of Medicine. Teeth and gum samples were collected at Saint Louis University’s dental school. Other samples came from Baylor College of Medicine in Houston, the other site that enrolled study participants.In all, the scientists identified more than 10,000 species of microbes that occupy the human ecosystem, documenting the impressive diversity of microbial life in the human body with more accuracy than earlier studies.Taking InventoryUsing new genomic techniques, TGI assistant directors Dr. Erica Sodergren and Dr. Makedonka Mitreva, took an inventory of the microbes in the samples. One approach involved sequencing a gene found in all bacteria. "This gene, 16S rDNA, serves as a barcode of life to indicate which species are there and their prevalence in the microbial community," says Dr. Sodergren. Another approach included sequencing the DNA of entire microbial communities in a subset of samples. "With this information, we could identify viruses, fungi and other non-bacterial organisms and put together a catalog of all the genes present in the samples," Dr. Mitreva says.The researchers noted unique communities of microbes in every site in the body. Interestingly, the microbial communities that live on the teeth are different from those in saliva. And the most diverse collection of microbes was found to live on the skin, which might be expected because it is the body’s barrier to the outside world.The scientists also reported that the body’s collection of microbes contributes more gene activity to human health than humans themselves. While the human genome includes some 22,000 genes, it’s a mere fraction of the 8 million genes that are part of the human microbiome.Genes Critical to HealthThese microbial genes are critical to good health. Those in the gastrointestinal tract, for example, allow humans to digest foods and absorb nutrients that our bodies otherwise could not handle. Microbial genes also produce vitamins and compounds that naturally suppress inflammation in the intestine.Also, confirming earlier, smaller studies, the new research shows that components of the human microbiome clearly change during an illness. When a patient is sick or takes antibiotics, the species of the microbiome may shift substantially as one bacterial species or another is affected. Eventually, however, the microbiome settles into a steady state, even if the previous composition is not completely restored.As part of the Human Microbiome Project, the NIH funded a number of studies to look for links between particular communities of microbes in the body and illness. Results of some of this research, reported in PLoS, underscore the clinical applications of microbiome research to improve human health.Unexplained FeverAt Washington University, researchers led by Dr. Gregory Storch, Professor of Pediatrics, examined the microbes in the noses and blood of children who developed sudden, high fevers that couldn’t be traced to a specific cause. Unexplained fever is a common problem in children under age 3, and they are often treated with antibiotics as a precaution, which contributes to antibiotic resistance.Dr. Storch and his colleagues, including Dr. Kristine Wylie, a postdoctoral research associate at The Genome Institute, found that specimens from the sick kids contained more total viral DNA and more species of viruses, some of them potentially novel, than children without fever, who also were included in the study as a comparison.“We found things we did not expect to see,” says Dr. Wylie, including the discovery of astrovirus in the children with fever, a viral type that causes childhood diarrhea and one that had not been previously found in blood. The researchers also showed that children without fever carried viruses, though in lower numbers. They plan to follow up this work with the most extensive characterization of viruses to date of healthy people. Understanding the difference between viral infections with and without fever will be important in applying microbiome techniques in the clinic, the scientists say.New Unnamed MicrobesIn another project, Dr. Weinstock, Dr. Wylie and their colleagues, along with Dr. Katherine Pollard and her team at the University of California, San Francisco, identified previously unknown types of microbes in stool samples from 11 healthy individuals. Stool samples are among the most well-characterized of any from the Human Microbiome Project, yet the group was still able to discover new bacteria. While these new, not-yet-named microbes were found in relatively low levels, the research indicates they may be quite common because they were found in multiple volunteers.Life-Threatening Illness in PremiesAnd in research at St. Louis Children’s Hospital, Dr. Phillip Tarr, Professor in Pediatrics, and neonatologist Dr. Barbara Warner, and others are investigating whether a life-threatening gastrointestinal illness in premature babies is linked to microbes in the intestinal tract. Necrotizing enterocolitis affects about 10 percent of premature babies, usually in the first month of life, and is fatal in 15 percent to 30 percent of cases. The researchers are collecting stool samples from premature babies to identify and quantify differences between the microbial communities of the infants who develop the illness and those who don’t. This information may provide a foundation for developing ways to prevent or cure the illness.NIH has funded a number of other medical studies using HMP data and techniques that TGI is involved in, including: the role of the gut microbiome in Crohn’s disease; the skin microbiome in acne; and the urogenital microbiome in sexually transmitted infections.“The future of microbiome research is very exciting,” Weinstock says. “This large-scale effort will open doors in many areas of medicine to improve our understanding of good health and the treatment and prevention of disease.”All the data collected on the human microbiome is uploaded to a central repository called the Data Analysis and Coordination center (Human Microbiome Project DACC) and is available for free to the scientific community.

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